Range of Services
Technologies & Know how
Expression Systems
Production Capacities
Molecular Biology
High-Throughput Production
Protein Engineering
Refolding
Purification Methods
Protein Analytics
Other Analytical Services

Brochure download
Refolding   
For proteins expressed in E. coli as inclusion bodies, we have developed a custom matrix-based refolding approach (potentially compatible with cGMP production process). This refolding study is based on an advanced preparation of inclusion bodies combined with the screening of up to 128 conditions including:
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protein concentration
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buffer
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pH
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redox potential
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ionic strength
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divalent cations


Example of Refolding Project   
Human Metalloprotease MMP-12 Refolding
Morales R, Perrier S, Florent JM, Beltra J, Dufour S, De Mendez I, Manceau P, Tertre A, Moreau F,
Compere D, Dublanchet AC, O’Gara M, (2004), Journal of Molecular Biology, 341(4), 1063-1076

Human macrophage elastase (MMP-12) plays an important role in inflammatory processes
and has been implicated in diseases such as emphysema and chronic obstructive pulmonary disease (COPD). It is therefore an attractive target for therapeutic agents.
By using our protein engineering and refolding tools, we have designed a MMP-12 protease
domain compatible with X-Ray structure resolution.
Protein'eXpert SA - PX'Pharma SA - Zone Minatec Entreprises -
7 Parvis Louis Néel – BP50 F - 38040 Grenoble
Phone: +33 (0)438 023 650 - Fax: +33 (0)476 961 038